South Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Auricular Perichondritis and Multifocal Chondromata in an Egyptian Donkey with Droopy4111789910.21608/svu.2018.17899ENMohieHaridyDepartment of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptAbdelnasserAbdel-HadyDepartment of Surgery, Anaesthesiology and Radiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptSohaSolimanDepartment of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.Journal Article20180830A 9-years-old female Egyptian donkey presented with ear drooping, multiple subcutaneous firm nodules (3-7 mm) on the medial surfaces of both ears and intact skin. Furthermore, the ear pinnae were diffusely thickened with subcutaneous induration. The cartilaginous plates of the ear pinnae were intact. The nodular lesions consisted of chronic perichondritis and cauliflower-like outgrowths of hyaline chondrous tissue. Mononuclear inflammatory cells diffusely infiltrated in the subcutaneous tissue and involved the dermal papillae, the dermis and the margin of the cartilaginous outgrowths. Multifocal cauliflower-like chondrous outgrowths emerged from the inner surface of both ears and consisted of irregular lobules of hyaline cartilage with quite regular deeply basophilic chondrocytes and fibrous lobules. T-lymphocytes and macrophages diffusely infiltrated the dermis, and both T-cells and B-cells were densely infiltrated around newly-formed blood vessels in the subcutaneous tissue. On the basis of the gross, histopathological and immunohistochemical findings, chronic auricular perichondritis and multifocal chondromata were diagnosed for the first time in an Egyptian donkey.https://svu.journals.ekb.eg/article_17899_dff6108ebb06915dcf4a22105b85ef71.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Histomorphogenesis of Upper Beak in Muscovy Ducks (Cairina moschata)12241790210.21608/svu.2018.17902ENKamalAbdallaDepartment of Anatomy and Histology, Faculty of Veterinary Medicine, Assuit University 71526, Assuit, EgyptSalmaMohamedDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, EgyptFatmaMadkourDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, EgyptJournal Article20180813<span>The objective of the present work was to provide information on the histology and morphometry of the upper beak of developmental ages of Muscovy Ducks (Cairina moschata) by using forty-ninehealthy post-hatch Muscovy ducks of both sexes ranging from 1-60 days-old. The upper beak was a wide spoon-shaped in all examined birds. Its length measured 19.32 mm at one day old and increased to reach 65.52 mm at 60 days old. The upper beak rostrally formed a smooth circumscribed plate like a finger nail, therefore called the nail of the upper beak, the nail of the upper beak projected rostroventrally beyond the nail of the lower beak.Scanning by electron microscopy revealed that the free tip of the upper beak contained two transversely curved rows of smalldome shaped dermal papillae at one-day-oldstage. Later, 12-14 transversely curved rows of small papillae were observed behind the dermal papillae at 15 days old, which increased to 18-20 in numbers at 60 days old.In all examined age groups, the lateral edges of the upper beak have lamellae; the ventral parts of the rostrally situated lamellae directed caudally, but the caudally situated lamellae directed vertically. The dermis of the upper beak was formed of dense connective tissue containing numerous lamellated sensory corpuscles on both sides of the lateral edges of the upper beak</span>https://svu.journals.ekb.eg/article_17902_d2d117238e295ce132fb612f4400e10d.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Immunohistochemical Study on the Ruminal Wall of Adult Baladi Goats (Capra hircus)25321790510.21608/svu.2018.17905ENEmamA.Histology Department, Faculty of Veterinary Medicine, Benha University, EgyptAbugherinBAnatomy and Histology Department, Faculty of Veterinary Medicine, Tripoli University, LibyaJournal Article20180904A total number of eight Baladi goats (Capra hircus)of both sexes aged between 11-18 months-old were used to describe the histological structure of the ruminal wallin addition to investigate the immunohistochemical localization and distribution of ki-67, caspase 3 and vimentin. The results revealed no sex differences in the ruminal wall histology or immunohistochemistry.Histologically, the ruminal wall consisted of mucosa-submucosa, muscularis and serosa. The mucosaof the rumen was thrown into ruminal papillae that were coveredby keratinized epithelium. The submucosa was consisted of loose connective tissue lacking glands. Circular and longitudinal smooth muscle layers were the components ofmuscularis however, the rumen was surrounded externally by typical serosa. The immunohistochemical study was done using theavidin-biotin immunoperoxidase method. Ki-67 immunostaining was limited to nuclei of basal cells layer in the ruminal epitheliumwhich explained the importanceof ki-67 in epithelial cells proliferation as well askeratin biosynthesis. Caspase 3 immunostaining was localized in cytoplasm and nuclei of some epithelial cells that were going to apoptosis.The vimentinimmunostaining was widely spread in epithelial cells as well as,fibroblasts in propria and submucosa, and endothelia of blood vessels.This showed theimportance of vimentin asan intermediate filament protein. Detection of vimentin in the glial cells of enteric plexuses indicatedits supportive role in the nervous control of the rumen. Overall, the histological and immunohistochemical findings of this study explainedthe structure-function relationship of the rumen of Baladi goatshttps://svu.journals.ekb.eg/article_17905_540db7a180873ad28f449115b281e1b5.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201In vitro And In vivo Molecular Studies On Some Escherichia coli Serovars Isolated From Chicken33491984810.21608/svu.2018.19848ENRokayaElamaryDepartment of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, EgyptDina M. W.Shibat ElhamdAnimal health research institute, Qena, EgyptWaeilSayedDepartment of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, EgyptWesamSalemDepartment of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, Egypt0000-0003-0212-2143Journal Article20180920A total number of 16 <em>Escherichia coli</em> serovars were recovered from different colibacillosis diseased chicken organs. Characterization of the isolates was performed by biochemical and serological tests, biofilm formation, antimicrobial susceptibility assays, detection of virulence and antibiotic resistance genes by PCR. The biofilm formation (at OD<sub>595</sub>) of all <em>E. coli</em> isolates showed a similar trend. eaeA gene was detected in 3 serovars O2:H6, O26:H11 and O44:H18 while, hly gene was detected in 2 serovars O2:H6 and O144:H21. The gene encoding for stx2 was detected in 2 serovars (O55:H7 and O146:H21) while the gene encoding stx1 was not detected in any serovars. Furthermore, all serovars were carrying the encoding blaTEM. blaSHV and blaCTX were detected in 75% and 18.75% of isolates respectively, while blaOXA-1 was not detected in all serovars. Although all isolates were multi-resistant against most of <em>B</em>-lactams antibiotics, they were susceptible to 20 to 80 mg ml<sup>-1</sup> of garlic extract. Finally, we demonstrated that a five-oral administration of garlic extract to baby chicks colonized with <em>E. coli</em> reduces the mortalities and significantly increase the body weight. The efficacy of garlic extract as the antibacterial natural product was sufficient as a bactericidal alternative against the multidrug- resistant <em>E. coli</em> isolates.https://svu.journals.ekb.eg/article_19848_125a90f4c9bb785545bbd231aa2190ba.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Complete Cervico-vaginal Prolapse (CVP) in a Ewe50591985510.21608/svu.2018.19855ENAhmed SAHassaneenDepartment of Theriogenology, Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, South Valley University 83523, Qena, Egypt0000-0003-1622-3623Journal Article20181028<span lang="EN-US">A four-years-old pluriparours (parity; n=2) pregnant Kanze/Dershawy ewe, of a body weight about 40 kg at Halayeb city, Red Sea province, Egypt was presented with a history of restlessness, straining, and appearance of a permanent/complete cervico-vaginal prolapse as a smooth, red, and rounded mass at the rear end of the ewe. There was no history of previous cervico-vaginal prolapse during or after previous pregnancies. Complete procedures of the appropriate successful treatment were described in this study. Briefly, treatment was performed under caudal epidural analgesia; </span><span lang="EN-US">the prolapsed mass was immediately covered with a towel soaked in water, examined for presence of injuries/rupture, raised to evacuate the urinary bladder, replaced by manual fist pressure and by the flat of hands, and finally retention suture was used to prevent the possible reoccurrence. The suture material had been removed before lambing, and the owner was advised to cull the ewe or at least excluded from further breeding.</span>https://svu.journals.ekb.eg/article_19855_5fce44db59bb3d57da115a85d9abad06.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201A study on Calf’s Behavior and Growth Performance in Relation to Gestation Period and Lactation Season60681985710.21608/svu.2018.19857ENHesham H.MohammedDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptMohamed Y.IbrahimDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptAl-Sadik K.SaleemDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptAzharFakhryDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptIbrahimYounissInspection Department of KomOmbo Veterinary Services, Aswan Governorate, EgyptJournal Article20181023<span lang="EN-US">The present study aimed to assess the impact of the length of the gestation period (GPL) and number of lactation season of cows on behavior and growth performance in calves from birth until weaning. The presence of abnormal behavior during 1<sup>st </sup>twelve hours of the calf's life as delayed Sternal recumbancy and standing or abnormal suckling was not significantly</span><span lang="EN-US"> affected by GPL.</span><span lang="EN-US"> Also, the duration and frequency of behavior related to respiratory, digestive or mixed disorders had a non-significant variation between groups of different gestation period length. However, the increase in GPL had </span><span lang="EN-US">the lowest percentage </span><span lang="EN-US">and duration of behavior related to respiratory and digestive</span><span lang="EN-US"> disorders. <span>Calves with the longest GPL had a significantly higher birth weight and it had a significant effect on pre-weaning growth rate. </span></span><span lang="EN-US">On the other side, the presence of abnormal behavior during the first twelve hours of the calf's life as delayed Sternal recumbancy and standing or abnormal suckling were higher in 3<sup>rd</sup> parity. The number of lactation season had no significant effect on the duration and frequency of behavior related to respiratory, digestive or mixed disorders. The birth weight of calves was higher in third parity group (36.73 kg) than other parities, but the differences didn't reach the significance, moreover, the </span><span lang="EN-US">weaning age and </span><span lang="EN-US">pre-weaning growth rate did not affect by the lactation season number. There was a negative correlation between abnormal behavior and the pre-weaning growth rate</span><span lang="EN-US">.</span><span lang="EN-US">There was a positive correlation </span><span lang="EN-US">between duration of abnormal behavior and</span><span lang="EN-US"> the weaning age.</span>https://svu.journals.ekb.eg/article_19857_1df6add904500d50db3e2af972dd1417.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Developmental Studies of the Gastric Junctions of the Post-hatching Muscovy Duck (Cairina moschata)69841986010.21608/svu.2018.19860ENFatmaMadkourDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, Egypt0000-0002-5641-9260Salma A.MohamedDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, EgyptKamal E. H.AbdallaDepartment of Anatomy and Histology, Faculty of Veterinary Medicine, Assuit University 71526, Assuit, EgyptYasser A.AhmedDepartment of Histology, Faculty of Veterinary Medicine, South Valley University 83523, Qena, EgyptJournal Article20180924<span lang="EN-US">The current study was carried out on 24 apparently healthy post-hatching Muscovy ducks of both sexes ranging from 1-60 days old. They were divided into 5 age groups to investigate the development of the junctions of the stomach; esophago-proventricular junction, p</span><span lang="EN-US">roventriculo-gizzard junction (Isthmus gastris)</span><span lang="EN-US"> and ventriculo-duodenal junction by using light and scanning electron microscopy. The longitudinal folds of the esophago-proventricular junction were separated by longitudinal grooves and they were crossed by thin transverse ridges; some of the longitudinal folds were branched toward the proventriculus at 60 days old. At one day old, the lymphoid infiltrations were observed in the lamina propria at the esophago-proventricular junction. With the advancement of the age, abundant diffused lymphoid infiltrations and esophageal tonsil were observed.</span><span lang="EN-US"> In all age groups, the cuticle covering of the mucosa at proventriculus-gizzard junction increased in thickness toward the gizzard. Moreover, in one day old duckling, the proventricular glands were observed underneath the gizzard tubular glands, these glands were become well developed at 60 days old.</span><span lang="EN-US"> The lamina propria of the gizzard-duodenal junction showed slight lymphatic infiltrations at one day old age. Lymphatic infiltrations and more lymphatic aggregations were abundant at 7-15 days. While between 30-60 days age, lymph nodules were distinct. Numerous endocrine cells were also observed in mucosa of the gizzard-duodenal junction.</span>https://svu.journals.ekb.eg/article_19860_9e0bb9ae9aa47826e6a4be5f88618af1.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Effect of Epidermal Growth Factors (EGF) on the Maturation and Developmental Competence of Buffalos’ Oocytes and Embryo Stages In Vitro85941987010.21608/svu.2018.19870ENNasra A.YousefDepartment of Theriogenology, Faculty of Veterinary Medicine, South Valley University, 83523, Qena, EgyptHassan A.HusseinDepartment of Theriogenology, Faculty of Veterinary Medicine, Assuit University,71515, Assuit, EgyptMagdy R.BadrAnimal Reproduction Research Institute (ARRI), ELHaram, Giza, EgyptAhmedEzzatDepartment of Theriogenology, Faculty of Veterinary Medicine, South Valley University, 83523 , Qena, EgyptBiology Department, Faculty of Science, King Khalid University, 61413 Abha, Saudi
Arabia.Journal Article20180927The present study aims to study the effects of epidermal growth factors (EGF) supplementations to the in vitro maturation (IVM) medium of buffalo's oocytes on the oocyte maturation rate and embryo development competence. The ovaries were collected from El-Monieb slaughters house and transferred directly to the laboratory for the experiment processing. Excellent and good cumulus oocytes complexes (COCs) were aspirated from 3-8 mm diameter follicles. TCM-199 medium were used for in vitro maturation. In experiment I, COCs were incubated in EGF-containing IVM medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs at 38.5°C in humidified environment; 5% CO<sub>2</sub> and 95% humidity. In experiment II, COCs were incubated in vitro fertilization (IVF) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. In experiment III, COCs were incubated in vitro culture (IVC) medium as; 0 (control or CTL), 10, 20 and 50 ng/ml EGF for 24 hrs in the same condition. According to the expansion of oocytes and attaining the developmental embryo stages of morula and blastocyst, our results have shown that addition of 20 ng/ml of EGF to the IVM medium significantly increased the oocyte maturation and fertilization rates compared to those of CTL (76.96 <strong>±</strong> 9.04 vs. 51.3 ± 4.66 and 67.96 <strong>±</strong> 2.76 vs. 42.03 ± 4.83 %, respectively) (P<0.05). On the other hand, Addition of 20 ng/ml of EGF to the IVM medium significantly increased the fertilization rates compared to other concentrations (10 and 50 ng/ml) (67.96 <strong>±</strong> 02.76 vs. 49.43 <strong>±</strong> 03.67 and 34.70 <strong>±</strong> 01.40, respectively). However, the developmental rates to morula and blastocyst stages in response to addition to IVC medium variably increased rather than those of control despite no significance. https://svu.journals.ekb.eg/article_19870_5a5a612590864ad3333aa6f4405ae386.pdfSouth Valley Univesity, Faculty of Veterinary MedicineSVU-International Journal of Veterinary Sciences2535-18261220181201Pathophysiology of Cholestatic Liver Diseases: New Insights into the Mechanisms of Bile Infarct Formation951052216210.21608/svu.2018.22162ENRehamHassanLeibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.Abdel-latifSeddekDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, EgyptAhmedGhallabLeibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.Journal Article20181216<span lang="EN-US">Cholestatic liver diseases can be induced for many reasons including obstructions, e.g. by stones or tumors. An early consequence of obstructive cholestasis is the formation of bile infarcts, which refer to clusters of dead hepatocytes due to bile salt accumulation. Although these infarcts were described long time ago (in 1876 by Charcot and Gombault), the leading mechanism is still unclear. Some hypotheses suggested direct killing by accumulation of bile salts up to toxic levels. Others claim indirect cell death via immune cell infiltration and inflammatory cytokine release. However, the sequence of events leading to the formation bile infarcts are still unclear. In the recent issue of <em>Hepatology</em>, Ghallab and his colleagues have recorded in a time-resolved manner the key events leading to bile infarct formation and the subsequent systemic changes, using two-photon based intravital imaging. This mini-review highlights the results of this study and discuss the time-resolved events in acute and chronic cholestasis, as well as the link between biliary bile salts and hepatocyte death. </span>https://svu.journals.ekb.eg/article_22162_9ee5b69e8c0e78ee06b2d246b417007d.pdf