Modulation of Glucose Homeostasis in Rats Treated with Bacterial Lipopolysaccharide: Role of L-Arginine Counter-Strategy

Document Type : Research article

Authors

1 Physiology department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt

2 General Authority for Veterinary Services, Ph.D in Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt

Abstract

The purpose of this study was to assess the effect of L-arginine on pancreas function in rats treated with LPS. Therefore, rats were weighed, randomly divided into 4 groups, and intraperitoneally injected as follows: Control group (saline), LPS group, L-Arg group (for 7 days), and LPS+L-Arg group (for 7 days then once injected with LPS). Serum IL-6, glucose, and insulin levels were assessed at 6, 12, 24, and 72 hours after last injection. Histological examination of the pancreas tissue was performed. Serum IL-6 elevated significantly 6 and 12 hours after LPS injection and retrieved at 24 and 72 hours. Association of LPS and L-Arg potentiate IL-6 production at 6 and 12 hours higher than that in L-Arg-treated group. Serum glucose was declined in single LPS-treated rats 6 and 12 hours and recovered at 24 and 72 hours after LPS injection. On contrary, Serum glucose were elevated 6 and 12 hours and declined near to control level 24 and 72 h after L-Arg injection. However, insulin levels were slightly elevated 12, 24 and 72 hours after LPS injection and 12 hours after LPS+L-Arg injection the rescued around the normal values at 24 and 72 hours. On contrary, serum insulin level decrease at 6 hours after L-Arg injection then recovered to control level. Histologically, rats treated with LPS showed necrosis, vacuolation in islets of Langerhans with infiltration of inflammatory cells. Pre-treatment of L-Arg prior LPS attenuated the alteration in pancreas tissue architecture however, rats treated with L-Arg showed normal pancreas tissue.

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