Clinicopathological and Hematological Changes in Consequence to Experimental Infection of Rabbits with Pasteurella Multocida Type A
Fatma
Mohamed
Poultry Diseases, Assiut Regional Laboratory, Animal Health Research Institute, Agricultural Research Center (ARC), Egypt
author
Abeer
El Hendy
Pathology Department, Assiut Regional Laboratory, Animal Health Research Institute, Agricultural Research Center (ARC), Egypt
author
Ahmed
Hassan
Department of Poultry and Rabbit diseases, Faculty of Veterinary Medicine, Assiut University
author
text
article
2020
eng
The current research aimed to study clinical, hematological and pathological changes occurred due to experimentally challenged rabbits with Pasteurella multocida type A. Twelve healthy Pasteurella multocida free rabbits were divided into control and challenged groups. Rabbits of the challenged group were intra-nasally inoculated with 0.5 ml of 1x105 CFU of Pasteurella multocida type A, while rabbits of the control group were inoculated with 0.5 ml of brain heart infusion broth (BHI). Clinical signs, hematological parameters and histopathological lesions were recoded for 14 days. Rabbits of challenged group showed nasal rubbing with minimal mucus discharge, conjunctivitis and reduction in spontaneous activity, but no mortality occurred. Hematologically, there was highly significant low RBCs count with concomitant significantly reduction of PCV in challenged rabbits, but hemoglobin concentration was insignificantly low in challenged animals as compared to control. Although, there was insignificant increase of WBCs count in challenged group, neutrophils and monocytes recorded significant and highly significant high value in challenged rabbits than control, respectively. Challenged rabbits, however demonstrated highly significant lymphopenia, but other blood parameters did not show any significant differences between challenged and control rabbits. Histopathologically, challenged animals showed meningitis, congestion and degenerative changes in respiratory epithelium, bronchopneumonia, vacuolar degeneration of hepatocytes and vascular changes in heart and spleen. Conclusively, the current study verified that challenging rabbits with Pasteurella multocida serotype A was not lethal and although it was essentially pneumotropic in nature, it caused lesions in other visceral organs, which in absence of bacteremia, could be caused by bacterial endotoxin liberated during disease pathogenesis.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
1
13
https://svu.journals.ekb.eg/article_87607_7b6e269459a00213167dc235bce826c7.pdf
dx.doi.org/10.21608/svu.2020.28129.1048
In vitro effect of zinc oxide nanoparticles on Staphylococcus aureus isolated from Layer Chickens
Fatma
Mohamed
Poultry Diseases, Assiut Regional Laboratory, Animal Health Research Institute, Agricultural Research Center (ARC), Egypt
author
Aml
Bakheet
Poultry Diseases, Assiut Regional Laboratory, Animal Health Research Institute, Agricultural Research Center (ARC), Egypt
author
Usama
Mahmoud
Department of Animal and Poultry Behaviour and Management, Faculty of Veterinary Medicine, Assiut University 71515, Assiut-Egypt
author
Omar A.
Amen
Department of Poultry Diseases, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut-Egypt.
author
text
article
2020
eng
This study aimed to evaluate the antibacterial activity of zinc oxide nanoparticles (ZnO-NPs) on Staphylococcus aureus (S. aureus) isolated from layer chickens using minimum inhibitory concentration test (MIC). A total number of 120-layer chicken samples from hock joints and livers were collected under aseptic conditions from diseased and freshly dead layer chickens from different private farms in Assiut Governorate. From these 120 samples, we isolated 29 isolates of Staphylococci with a prevalence rate of 24.16%. Nineteen (65.5 %) of these 29 isolates were coagulase negative and ten (34.5 %) were coagulase positive. By PCR, the 10 coagulase positive strains (CoPS), were confirmed to be S. aureus as they have (clfA gene), while 6 isolates only were Methicillin Resistant Staphylococcus Aureus (MRSA) as they demonstrated mecA gene. Nine isolates of S. aureus demonstrated variable antibiotic sensitivity patterns in MIC test against ZnO-NPs and some selected antibiotics. ZnO-NPs showed the best antibacterial efficiency against most isolates as compared to all tested antibiotics.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
14
24
https://svu.journals.ekb.eg/article_87620_6cda27f03023e554a1c50e17c1f433f6.pdf
dx.doi.org/10.21608/svu.2020.28106.1047
Evaluation of Xylazine HCl as a Potential Anesthetic for Laprogastrotomy Operation in Dogs
Shereen
Hussein
MVS of Surgery, Anesthesiology, and Radiology, Faculty of Vetrinary Medicine, Assiut University, Assiut, Egypt.
author
Ahmed
Ibrahim
Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
author
Enas
Noseer
Department of Biochemistry, Faculty of Vetrinary Medicine, Aswan University, Sahary city, Aswan 81528, Egypt.
author
Mohamed
Semieka
Department of Surgery, Anesthesiology, and Radiology, Faculty of Vetrinary Medicine, Assiut University, Assiut 71526, Egypt.
author
text
article
2020
eng
The present study aimed to evaluate intramuscular xylazine HCl 2% as an anesthetic for laparogastrotomy in dogs at different doses. Twenty-seven dogs were divided randomly and equally into three groups; group A received 4 mg/kg, group B, received 8 mg/kg, and group C received 14 mg/kg. Anesthetic indices, effect on physiological parameters, blood gases, and biochemical parameters were recorded. The onset was 4.00 ± 0.00, 3.50 ± 0.33, and 5.00 ± 0.33 min, and the duration was 18.00 ± 2.50, 21.00 ± 7.20, and 28.00 ± 4.30 min in the groups A, B, and C, respectively. Pedal reflex was absent in groups A and B from 5 - 30 min post-injection. Palpebral reflex was absent in all groups from 20 - 30 min post-injection. All dogs recorded a non-significant decrease in the rectal temperature, but a significant decrease in respiratory and heart rates. There was a significant increase in the PvCO2 level and significant hyperglycemia in all dogs. Dogs in groups B and C exhibited a significant decrease in PvO2. There was a significant decrease in the serum cortisol level in the group B. Creatinine level recorded non-significant decrease in the group A, while there was a non-significant increase in the group B. There was non-significant decrease in GGT levels in the groups A and C. Xylazine at dose of 14 mg/kg established a satisfactory stable anesthesia that was enough to conduct laparogastrotomy in dogs.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
25
37
https://svu.journals.ekb.eg/article_91023_61c4014cddf0d9c131dd1c8985ad8898.pdf
dx.doi.org/10.21608/svu.2020.29353.1051
Investigation the Prevalence of Common Parasitic Infections in Farmed Quails in Upper Egypt
Ahmed
Hassan
Department of Poultry Diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
author
Esam
Naeem
Assiut Veterinary Medicine Directorate, Assiut, Egypt.
author
Mohamed
Soliman
Department of Poultry Diseases, Faculty of Veterinary Medicine, El-menia University, El-menia, Egypt
author
text
article
2020
eng
The present study was conducted to investigate the prevalence and identification of most common parasitic infections of quails and to determine their seasonal variation. One hundred diseased quails (60 quails in summer and 40 quails in winter) were obtained from quail farms in different localities of Assiut and El-menia Governorates and were examined for the presence of ectoparasites, endoparasites, intestinal protozoa, blood protozoa and parasites of other organs (lungs, brain and liver). Results revealed that neither ectoparasites nor macroscopic parasites were observed in the examined quails. Mainly, the encountered parasites were intestinal protozoa (44%), blood protozoa (25%) and parasites of other organs (28%). The overall parasitic infection rate in the examined quails was higher in cold season (57%) than warm season (53%). The intestinal protozoa were identified including Eimeria spp. (30%), Cryptosporidium spp. (19%), Tetratrichomonas galinarum (15%), Cyclospora spp. (4%), Isospora spp. (3%) and Microsporidia spp. (3%). The prevalence rate of intestinal protozoal infection was higher in warm season 46.6% (28 out of 60 examined birds) than cold seasons 40% (16 out of 40 examined birds). The incidence rate of mixed intestinal protozoal infection was higher (24.8%) than the single infection (18.8%). The blood protozoa were reported including Leucocytozoon spp. (9%), Babesiosoma spp. (8%), Aegyptianella spp. (7%), Plasmodium gallinaceum (4%), Haemoproteus spp. (4%), Atoxoplasma spp. (1%) and Ehrlichia spp. (1%). The prevalence of parasitic infection in other organs showed that respiratory tract (trachea and lung) infection with Cryptosporidium spp. (24%), hepatic histomoniasis (3%) and brain toxoplasmosis (7%).
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
38
50
https://svu.journals.ekb.eg/article_98372_2e7f5c66acf0c6c029279139fdd9a0c2.pdf
dx.doi.org/10.21608/svu.2020.31915.1058
Survey of Toxoplasma gondii antibodies in retail red meat samples in Erbil governorate, Kurdistan Region, Iraq
Dhary
Almashhadany
Public Health and Bacteriology
Knowlege University, College of Science, Kurdistan Region, Iraq
author
text
article
2020
eng
Since livestock meat, has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of infection with T. gondii in these animals’ meat is needed to protect public health. Latex agglutination test (LAT) and enzyme-linked immunosorbant assay (ELISA) were performed on meat juice from 380 red meat samples (125 Beef, 135 Mutton, and 120 goats’ meat) that are sold in Erbil governorate, Kurdistan region, Iraq. The current results demonstrated that the overall prevalence of anti-T. gondii antibodies among red meat was 18.7% and 16.3% according to LAT and ELISA, respectively. The highest rate was found in October (25.8% and 22.6%) by using LAT, and ELISA, respectively. While, the lowest rate was recorded in August (12.5% and 9.4%) by both assays, respectively. Both tests performed similarly without significant difference between their performances as diagnostic tests. Moreover, no significant differences between meat types were found in terms of toxoplasmosis antibodies. In conclusion the current survey provides significant evidence about risk of human exposure to T. gondii through the consumption of raw or undercooked red meat potentially contaminated with infectious tissue cysts.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
51
59
https://svu.journals.ekb.eg/article_112829_d5199a04a64dde94ef1fc70828d2348b.pdf
dx.doi.org/10.21608/svu.2020.31892.1057
Therapeutic effectiveness of Ocimum basilicum extract on bovine cutaneous papillomatosis
AbdulRahman
Saied
Veterinarian at Ministry of Tourism and Antiquities - Aswan office
author
Ashraf A.
El-Ghoneimy
author
Abdel-latif
Seddek
author
Sary Kh.
Abdel-Ghafar
author
Samy A.F.
Morad
author
text
article
2020
eng
Bovine cutaneous papillomatosis is a common cutaneous disease of cattle in the Egyptian veterinary field. Ocimum basilicum L. (Rehan) is one of the aromatic plants originating in Asia and Africa. Many studies showed that Ocimum basilicum has interesting antiviral and anticancer activities. However, there is no report demonstrating the clinical significance of the anti-papilloma activity of Ocimum basilicum against bovine skin papillomas. Thus, our study was designed to evaluate the therapeutic potential of Ocimum basilicum extract (OBEx) as an anti-papilloma agent against bovine papillomatosis. OBEx was prepared and undergone phytochemical analysis that revealed presence of alkaloids, phenolics, and flavonoids. Ten cutaneous papillomatosis-infected cattle were diagnosed clinically and histopathologically. Animals were treated with OBEx 2% ointment that topically applied daily and papillomas regression was recorded weekly. Clinically, papillomas started to disappear from the 7th - 21th day after the start of treatment. Histopathological analysis showed improvement in histological features of wart tissue returning to the normal skin structure with presence of lymphocytic infiltration. We concluded that the topical application of OBEx is an effective, promising alternative, cheap, and easily apply agent for treatment of skin papilloma.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
60
77
https://svu.journals.ekb.eg/article_112992_f361db953597ecddbe234a99a89bd959.pdf
dx.doi.org/10.21608/svu.2020.35596.1067
Production of lactic acid from whey permeates using lactic acid bacteria isolated from cheese.
Waiel
Sayed
Department of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, Egypt.
author
Wesam
Salem
Department of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, Egypt
author
Zeinab
Sayed
Department of Botany and Microbiology, Faculty of Science, South Valley University, 83523 Qena, Egypt.
author
Abdelmoneim
Abdalla
Food Science Department, College of Agriculture, South Valley University, 83523 Qena, Egypt.
author
text
article
2020
eng
The uses of whey permeate for lactic acid production is a dual-purpose process by producing lactic acid and decreasing the environmental pollution problem caused by dumping the lactose- rich dairy byproduct. This study aimed to investigate lactic acid production from whey permeates using lactic acid bacterial isolates. Five isolates were identified as lactobacillus casei MT682513, Enterococcus camelliae MT682510, Enterococcus faecalis MT682509, Enterococcus lactis MT682511, and Wissella paramesenteroides MT682512 using 16S rRNA. Small scale batch fermentations of permeate were conducted under uncontrolled pH conditions. pH, temperature, carbon, and nitrogen sources as well as fermentation time on the conversion rate of lactose to lactic acid were monitored. It was found that Lactobacillus casei exhibited the highest percentage of lactic acid production without any supplementations. The increasing percentage was 107% using glucose (100 gl–1) and 44.2 % using yeast extract (10 gl–1) as carbon and nitrogen sources, respectively. The optimum lactic acid production was between 30°C and 37°C within a pH value of 6. The highest production of lactic acid under the optimized conditions resulted after 14h of fermentation. This work facilitates further studies of Lactobacillus casei over the optimized conditions of whey parameters on the other industrially important for lactic acid production and applications.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
78
95
https://svu.journals.ekb.eg/article_113480_a5d0574e763b88e9a2d0da51503da4dc.pdf
dx.doi.org/10.21608/svu.2020.35000.1064
Morphological features of pharyngeal roof of Egyptian geese (Alopochen aegyptiacus)
Mohammed
Abdelsabour-Khalaf
Department of Anatomy and Embryology
author
Yasser
Ahmed
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
author
text
article
2020
eng
The anatomy of the pharyngeal roof of Egyptian geese has been studied to define the structural features which may affect swallowing and food intake, in addition to provide a basis for bird pathology identification in this area. The results showed that the pharyngeal roof of geese was smooth with numerous scattered conical papillae of various sizes and had several openings of the sphenopterygoid salivary glands. Its length was 16.2 mm, about 16.5 percent of the total length of the oropharyngeal roof. A common opening of both ear tubes; infundibular cleft, was measured 8.09 mm and extended caudally to pharyngoesophageal junction through shallow groove. The pharyngeal mucosa contained abundant mucous and serous glands associated with lymphatic nodules, in addition to accumulation of nerve cells. In conclusion there were some anatomical features of the pharyngeal roof of geese that are unique to this species and morphological changes to this area of the digestive tract can reflect adaptations to the bird's environment and mode of feeding.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
96
105
https://svu.journals.ekb.eg/article_113906_edf9602752ba768619d787e2a1dda29a.pdf
dx.doi.org/10.21608/svu.2020.35503.1066
Histomorphogenesis of the Pharyngeal roof in Muscovy Ducks (Cairina moschata)
Fatma
Madkour
Department of Anatomy and Embryology of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
author
Kamal E. H.
Abdalla
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt.
author
Salma Ahmed
Mohamed
Department of Anatomy and Embryology of Veterinary Medicine, South Valley University, Qena 83523, Egypt
author
text
article
2020
eng
This study aimed to investigate the morphological characteristics of the pharyngeal roof of the developmental stages of Muscovy ducks from 1 to 60 days old. The statistical analysis revealed that the ratio of the pharyngeal roof to the total length of the oropharynx decreased with advancement of the age, which was recorded 19.44 % at one-day and 15.19% at 60 days age, as well as the infundibular cleft was increased in length approximately by one and half at 15 days and three folds at 60 days when compared with that of one-day-old. SEM in one-day-old showed that, the fine pharyngeal papillae on each side of the infundibular slit, whereas, two types of the papillae at 15 days but numerous different sized and shape caudally directed papillae at 60 days, some of them which were present at the pharyngoesophageal junction were in appearance of the nails of the carnivores. Numerous openings of the sphenopterygoid salivary glands were scattered between these papillae, which were observed in older ages (60 days old) by few numbers in the shallow groove at the caudal commissure of the infundibular slit. The edges of the infundibular cleft were smooth in all developmental stages except at 60 days old where few, fine and caudally directed papillae were recognized. Histologically, the sphenopterygoid salivary glands were compound tubule-alveolar mucous secretory glands which was surrounded by lymphatic aggregation in older ages. In all examined ages, lymphatic accumulation could be observed in the infundibular cavity which was surrounding the intraepithelial mucous glands.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
106
114
https://svu.journals.ekb.eg/article_114263_8978efd561720c06a03e6fe69b600ed3.pdf
dx.doi.org/10.21608/svu.2020.32880.1061
Comparative Histology and Micrometric Analysis of Pharyngeal cavity in Egyptian Laughing Dove (Streptopelia senegalensis aegyptiaca) and Japanese Quail (Coturnix coturnix japonicum)
Fatma
Madkour
Anatomy and embryology, vet.medicine,svu,Egypt
author
text
article
2020
eng
Birds of different families; Japanese Quail (family Phasianidae) and Laughing Dove (family Columbidae) were used for this study to focus on the histological and micrometric comparison of the pharyngeal roof and floor with its content. The thickness of the lining epithelium of the pharyngeal roof, root of the tongue was almost equal in both species, whereas of the laryngeal mound of quail was nearly 5 times thicker than that of dove. On the other hand, the diameter of the glandular lobules of all studied salivary glands in the pharyngeal cavity of quail was more than that of dove except caudal lingual salivary glands. The entrance of the infundibular cavity of dove was infiltrated by aggregations of lymphoid tissue, but there were variably sized lymph nodules in quail termed pharyngeal nodules. The sphenopterygoid salivary glands (branched tubular mucous type) of dove were fewer than those of quail. The caudal lingual salivary glands were concentrated centrally dorsal to basihyoid of the hyoid bone in dove but distributed dorsolaterally to the basihyoid in quail. The lamina propria of the laryngeal mound of dove had 2 groups of circopterygoid salivary glands (rostromedial and caudomedial), lining by tall columnar epithelium, while of quail had 3 groups (rostromedial, rostrolateral and caudomedial) circopterygoid salivary glands, lining by low columnar epithelium. The laryngeal mound of both species was supported by two groups of intrinsic laryngeal muscles and 3groups of extrinsic laryngeal muscles.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
115
129
https://svu.journals.ekb.eg/article_129075_fa33ac6bffef3da12a09668062879992.pdf
dx.doi.org/10.21608/svu.2020.36973.1071
Chemical and Sensory investigations of Some commercial and Home-made products
Taghreed M.
Galal
Home Economics Department, Faculty of Specific Education, Assiut University, Assiut Egypt
author
Seham A.
Farrag
Home Economics Department, Faculty of Specific Education, Assiut University, Assiut Egypt
author
Rofida F.
Moftah
Food Science and Technology Department, Faculty of Agriculture, Assiut University, Assiut Egypt
author
text
article
2020
eng
Food we eat is one of the main concerns of contemporary society and is increasingly attracting the attention of public institutions, When junk food is consumed very often, the excess fat, simple carbohydrates, and processed sugar found in junk food contributes to an increased risk of obesity, cardiovascular disease, and many other chronic health conditions. In addition to high prices for fast foods. Overall, our findings suggest that increases in the supply of fast food restaurants have a significant effect harmful. Therefore, in this study, we have produced home-made types of fast food, such as (Chicken burger sandwich, Beef burger sandwich, French fries, KFC and KZB sandwich). In this investigation, we performed chemical composition, fatty acid analysis, and sensory evaluation of both homemade and restaurant made sandwiches. It was noted that there is an increase in the content of protein, carbohydrates, fiber, and moisture. Moreover, there are a decrease in the content of fat and ash, the amount of calories as well as a decrease in the percentage of saturated fatty acids, in sandwiches prepared at home when compared to the ones prepared in the restaurant. Furthermore, it was found that it was an increase in the consumer's accepts in general than the products prepared in restaurants.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
3
v.
2
no.
2020
130
141
https://svu.journals.ekb.eg/article_249565_d282606fe283b6c7a7f2b4f3df4bdf75.pdf
dx.doi.org/10.21608/svu.2020.125414.1180