Hygienic and sanitary condition of environment and meat surfaces in the restaurant of Sohag University Hospital
Abeera
Mahmoud El-Sayed
Fellow- Sohag University Hospital, Sohag University, Sohag, 82524, Egypt
author
Motamed
Mahmoud
Department of Animal Behavior and Husbandry (Genetics, Breeding and Production), Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt,
author
Sotohy
Sotohy Mohamed
Dept. of Animal & Environmental Hygiene, Faculty of Veterinary Medicine, Assiut University
author
text
article
2021
eng
Background: The microbiological quality of red meat produced from most of the food-processing plants in Egypt has always been questionable. Aim of work: This study aimed to examine the bacteriological quality of a restaurant in the Sohag University hospital environment (air, water; worker's hands, worker's clothes, and knives) beside the meat surfaces. Methods: Bacteriological examination was performed for air, water, worker's hands, worker's clothes, and knives, in addition to the meat surfaces. Mean total bacterial count; TBC, total coliform count; TCC, total fecal coliform count; TFCC, total Escherichia coli count, and total Staphylococcus aureus were carried out. Results: The obtained results revealed that the TBC, TCC, TFCC, and total E. coli counts were higher than the recommended standard for sanitary practices. In addition, we observed that worker's clothes contain more bacterial count than the hands and knives. The knives' swabs contained less bacterial burden but still higher than the recommended guidelines. In addition, 9 bacterial isolates were consistently isolated during this study including; E. coli, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeroginosa, Enterobacter spp, Klebsiella spp., Proteus spp, Citrobacter spp, and Serratia spp. with varying percentages of frequency across the sampling points. Whereas, none of Salmonella spp. were isolated. Conclusion: the presence of pathogenic microorganisms in this study is of special concern and meat Hygienists should be fortified to review the processes involved in the environment surrounding the meat as well as meat processing of University restaurants in Egypt.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
1
11
https://svu.journals.ekb.eg/article_181732_0e6dac1c590e0ecf1265ccf055581515.pdf
dx.doi.org/10.21608/svu.2021.65185.1116
Potential of hydroponic barley in rabbit diets: effect on productive performance, nutrient digestibility, microbiological and physiological responses
Esraa
Mohamed
Department of Animal and Poultry Production, Faculty of Agriculture, South Valley University, Qena 83523, Egypt
author
Hamdy
Hassan
Department of Animal and Poultry Production, Faculty of Agriculture, South Valley University, Qena 83523, Egypt
author
Ahmed
Abdel-Wareth
Department of Animal and Poultry Production, Faculty of Agriculture, South Valley University, Qena 83523, Egypt
author
text
article
2021
eng
Application of agriculture hydroponic technology with a view to reduce the gap between the animal nutritional requirements and availability of feed ingredients create an important role in animal and rabbit production. Hydroponic fodder production such as barley is an alternative technology to cultivate fodder which provide the growing nutrients requirement for livestock feed with suitable prices, high quantity, a short growth period, a small land for production, a clean environment, water efficiently and reduces cultivate fodder resources. This study was investigated with view to give an overview on the possible role of hydroponic barley (HB) as a feed replacement in rabbit ration. Various scientific published research articles were used HB as a feed replacement in rabbit ration. The most observed and analyzed productive and reproductive performance, nutrient digestibility and physiological responses. Positive effect of feeding HB was observed on serum metabolic profile and microbial count of caecum of rabbits. However, the economics and nutritive values of hydroponic production system should be carefully estimated. This review provides an overview of and illustrates the positive effects of HB as a feed replacement in rabbit diets and their potential effects on productive performance, economic return, nutrient digestibility, microbiology and gut health.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
12
23
https://svu.journals.ekb.eg/article_189426_86c5a1c1ce48f886b4751671c9bc97ff.pdf
dx.doi.org/10.21608/svu.2021.67695.1114
Molecular characterization of sheep major histocompatibility complex (OVAR-MHC) Class II DQA1 gene in two Egyptian breeds
Amr
Mohamed
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
Hemely
Hassan
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
Fayza
Aly
Department of Zoology, Faculty of Science, South Valley University, Qena, Egypt
author
Alsagher O.
Ali
Department of Animal Medicine, Division of Infectious Disease, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
author
text
article
2021
eng
The major histocompatibility complex (MHC) is a large locus on vertebrate DNA that contains a set of closely related polymorphic genes coding for cell surface proteins essential for an adaptive immune system. MHC is thought to be under strong selection pressure because of its main role in infectious disease resistance. Thus, the pattern of different MHC genes variation should reflect this selection pressure in animal's natural populations. To test this hypothesis, we investigate the nature and frequency of polymorphism of the sheep MHC class II DQA1 gene in two different common local Egyptian sheep breeds (Ossimi and Saidi). Ovar-DQA1 gene is considered one of the most important response genes in the MHC region. In the current study, blood samples of 50 Egyptian sheep from two breeds (32 Ossimi and 18 Saidi) with different ages and sex and from seven different farms in Qena governorate were collected for DNA extraction and PCR amplification. The PCR products of 18 DQA1 alleles were examined using direct sequencing, for the detection of polymorphism in a functionally relevant domain of the DQA1 gene. Among the investigated samples the phylogenetic tree reconstruction and sequence comparison revealed different DQA1 alleles. Thirty-two sheep samples lacking DQA1, five different alleles were identified for the two breeds. The multiple sequence alignment illustrates nucleotide polymorphism and conserved regions in the identified sequences. This variation leads to a better understanding of the genetic makeup of Ovar-MHC and facilitates the selective breeding programs to determine the resistance and susceptibility to disease in sheep.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
24
37
https://svu.journals.ekb.eg/article_189665_f3b046adf21443e4d34123dd1d9d52a2.pdf
dx.doi.org/10.21608/svu.2021.75495.1125
Experimental and clinical evaluation of a new intra-lingual suture pattern for management of lingual wounds in farm animals
Aiman M.
Seddek
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Sohag University, 82524 Sohag, Egypt.
author
Mohammed H.
Elrashidy
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Sohag University, 82524 Sohag, Egypt.
author
Sayed F.
El-Hawari
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Sohag University, 82524 Sohag, Egypt.
author
text
article
2021
eng
A pre-clinical experimental study is used to evaluate a new intralingual suture pattern, for treating farm animal’s tongue-wounds. Experimental section was performed on 36 fresh goat’s tongue-samples, to compare the new suture pattern with the traditionally used two patterns (double rows simple interrupted pattern; and tension relieve vertical mattress with deep row simple interrupted pattern that is followed by superficial opposing vertical mattress). Comparison involved the required stitches number, duration of suturing, and pattern’s tensile strength. The main clinical study was carried out on recent lingual wounds of variable depths which involved 30-50% of tongue width in one horse, one camel, one buffalo-calf, and three donkeys. In addition to three donkeys had old lacerations which failed to heal by first intension. Wounds sutured by the new pattern were covered by one row of cruciate mattress to oppose the mucosa. Results revealed that the new pattern required fewer stitches numbers, consume lesser time, had higher tensile strength, it could be applied easily, achieved proper apposition of both the muscular layer and mucosa, and it induced minimal scar formation and deformity. The technique is widely acceptable by the owners than the other traditional merhods. The technique is advised to be used as alternative suture pattern for repair of lingual wounds of farm animals.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
38
50
https://svu.journals.ekb.eg/article_192878_21cdea5c916e588ad4f628524070aafd.pdf
dx.doi.org/10.21608/svu.2021.75493.1126
Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation—Pivotal Role of COX-2 Signaling
wael
Twafik
Department of Biochemistry, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt
author
Osama
Morad
Neurology and Psychological Medicine department, Faculty of Medicine, Sohag University
author
Reham
Hassan
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
author
Khaled
nematallah
Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt (BUE), Cairo,
author
Dina
El-hamed
Poultry Research Department, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt
author
Samy
Morad
Department of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
author
Noha
Abdelmageed
Department of Pharmacology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
author
text
article
2021
eng
The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascular- permeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX-2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
51
69
https://svu.journals.ekb.eg/article_193550_4b77f674445ac3550bdffc57ba6067fa.pdf
dx.doi.org/10.21608/svu.2021.70729.1119
Bone exostosis lesions in distal limb of equines (17 cases; 10 horses, 7 donkeys): A clinical study.
Mohammed A.
Noby
Department of Surgery, Anesthesiology, and Radiation, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt
author
Abdel-Nasser A.
Abdel-Hady
Department of Surgery, Anesthesiology, and Radiation, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt
author
Ahmed S.
Soliman
Department of Surgery, Anesthesiology, and Radiation, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
author
Elhussein E.
Mahmoud
Department of Surgery, Anesthesiology, and Radiation, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt
author
text
article
2021
eng
Lameness is an important clinical sign for the problems of locomotor system. When, visual inspection and diagnostic analgesia are not enough for reaching out the accurate diagnosis of the lesion, diagnostic imaging including radiology helps to determine the problem in a specific area. This study investigated the role of radiography as a crucial step for clinical diagnosis of bone exostosis lesions in equines. Between July 2019 and December 2020, seventeen animals were involved in this study 10 horses and 7 donkeys. These animals (age, 4.1±3.8 years; weight, 199±98 Kg) were suffered from lameness with prominent bone exostosis on the distal limb, then were subjected to radiographic examination with lateromedial and dorsopalmar/dorsoplantar views. The most detected cases were more prevalent in the fore limb (76.5%) than the hind limb (23.5%). The most prevalent lesions recorded in this study were ring bone (9 cases; 52.9%), Degenerative joint disease of the carpal joint and sore shins (3 cases; 17.64%) for each, and then bone spavin and side bone were the lowest prevalent one (1 case; 5.88%) for each. There was no treatment for all cases except sore shins which was improved thoroughly by topical iodine compounds with systemic administration of phenylbutazone. X-ray radiography is still beneficial for equine industry especially in the lesions related to the joints and bone exostosis that appeared on the distal limb.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
70
79
https://svu.journals.ekb.eg/article_193617_378356a9fe0298f1e45e85f6ee71a20b.pdf
dx.doi.org/10.21608/svu.2021.72294.1121
Gross anatomical and morphometrical study of the nasal cavity (cavum nasi) of Egyptian goat (Capra hircus) and Egyptian Baladi dog (Canis lupus)
Fatma
Madkour
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt.
author
Mohammed
Abdelsabour-Khalaf
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt.
author
text
article
2021
eng
This study provides sufficient information on the anatomical investigation and the morphometrical analysis of the nasal cavity of Egyptian goat and Egyptian Baladi dog. The sagittal and the cross (transverse) sections at the different levels were performed on twenty heads of those animals. The nasal cavity was typically cone-shape in goat and a slight narrow elongated cone-shape in dog. It was completely divided by the nasal septum in both species except the caudal part of the nasal cavity of goat was partially divided. The nostril of the goat was nearly horizontal slit-like in form, directed ventromedially toward the philtrum. Whereas, the nostril of the dog was somewhat comma-shaped with its wide part directed medially toward the nasal septum. In the sagittal section, the nasal conchae were included in goat and dog, the dorsal nasal concha, the ventral nasal concha, the middle nasal concha and the ethmoidal conchae. The dorsal nasal concha was the longest concha in both animals. In the cross section, the dorsal nasal concha in goat was formed the dorsal conchal sinus at the level of the third premolar teeth and caudally extended into the frontal sinus. The ventral nasal concha was not formed sinus but its spiral lamellae enclosed the recesses. Moreover, the middle conchal sinus was appeared at the level of the first molar teeth. In this section, all the nasal conchae of dog were formed recesses (conchal sinuses absent).
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
80
93
https://svu.journals.ekb.eg/article_194039_a57277549d3000e8e155172a7ae9db3d.pdf
dx.doi.org/10.21608/svu.2021.86098.1134
The effect of CpG-ODN (Toll-like receptor 21 agonist) as an adjuvant for Newcastle disease vaccination in broiler chicken
Ghada K.
Gamal
Master student in Avian and rabbit diseases, faculty of veterinary medicine, Assiut University, Assiut, Egypt.
author
Mostafa
Shehata
Department of Avian and rabbit diseases, faculty of veterinary medicine, Assiut University, Assiut, Egypt
author
Shiem
El-Sherry
Department of Avian and rabbit diseases, faculty of veterinary medicine, Assiut University, Assiut, Egypt
author
text
article
2021
eng
The present study was conducted to evaluate the immune-stimulating effect of CpG-ODN (Toll-like-receptor-agonist21) on broiler chickens vaccinated against Newcastle disease. The motifs were examined with different vaccination programes using different inoculation routes. 270 (one-day-old) - chicks were divided into 6 groups. Group one as a negative control sham inoculated with PBS; group two was injected intramuscularly with CpG-ODN; group three was vaccinated with live ND vaccine by eye-drop route; group four was vaccinated by eye-drop route and injected with CpG-ODN; group five was vaccinated with Newcastle disease inactivated vaccine and injected intramuscularly with CpG-ODN; group six was vaccinated by eye-drop route with live attenuated vaccine dissolved in CpG-ODN. Antibodies responses were monitored based on hemagglutination inhibition test and ELISA assay for the detection of IgG. The average body weight gain and feed conversion ratio were estimated to evaluate CpG-ODN growth-promoting effect. All vaccinated groups supplied with CpG-ODN recorded higher antibodies titer than the only vaccinated group. Group 4 which vaccinated and inoculated intra-muscularly with CpG-ODN recorded higher antibodies titer than other vaccinated groups. Antibodies titer was similar in group five and six without significant difference and both were lower than group 4. ELISA assay confirmed the significance between the adjuvanted groups at 21 and 35 days old. There were no significant differences (p≥0.05) observed between the vaccinated and adjuvated groups in either average body weight gain or in feed conversion rate. In conclusion, CpG-ODN proved its efficiency as an adjuvant when co-inoculated with Newcastle disease vaccines via different administration routes.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
94
102
https://svu.journals.ekb.eg/article_197058_599ff8c3fef3ed78d964a8e5a5321296.pdf
dx.doi.org/10.21608/svu.2021.79758.1129
Morphological and molecular characterization of Sarcocystis cameli and Sarcocystis ippeni from the muscles of One-Humped Camel (Camelus dromedarius ) in New valley Governorate, Egypt.
soheir
rabie
Zoology Department, Faculty of Science, South Valley University, Qena, Egypt
author
Reda
Hassanine
Former professor;, Zoology Department, Faculty of Science, New valley University, EL-Kharga, Egypt
author
amal
A. hassan
Zoology Department, Faculty of Science, Damnhour University, Damnhour, Egypt
author
obaida
Abo Elhussien
Zoology Department, Faculty of Science, South Valley University, Qena, Egypt
author
Mohammed
B. M. EL-Mahdi
Zoology Department, Faculty of Science, South Valley University, Qena, Egypt
author
text
article
2021
eng
Sarcocystis spp. are cysts-forming coccidian parasites which infect several animals including camels as intermediate hosts. The present study was designed to study the Sarcocystis infection in camels (Camelus dromedaries) at morphological and molecular levels. Sample of the esophageal, heart and ocular muscles tissue were collected from infected camels from El-kharga (New valley Governorate) and examined for Sarcocystis spp. using macroscopic evaluation, light microscopy (LM), transmission electron microscopy (TEM) and molecular analysis. Two species Sarcocystis cameli and Sarcocystis ippeni were recognized. Sarcocysts were thin walled with barely visible projections. Using the TEM, the two structurally distinct sarcocysts were recognized by unique villar protrusions (Vp). Sarcocysts of S. cameli had Vp of type 9j. The sarcocyst wall had upright slender Vp, up to 0.1-0.4 μm long and 0•05-0.1 μm wide. On each Vp, Rows of knob-like protrusions are present appeared to be interconnected. Sarcocystis ippeni had ‘‘type 32’’ sarcocyst wall characterized by conical Vp with an electron dense knob. The Vp were approximately 175.27-266.76 nm long, 100.45-175.98 nm wide. In each Sarcocystis, the Vp had microtubules (Mt) that originated at midpoint of the ground substance (Gs) and continued up to the tip. Molecular data revealed the amplification of partial fragments of the 18S rRNA gene (~600 bp). The digestion analysis of obtained PCR products using RFLP method utilizing (Mbo1) appeared 2 bands approximately 250, 350 bp for each Sarcocystis. Molecular analysis demonstared the ability of 18S rRNA gene for distinguishing Sarcocystis species in studied animals and to be used as molecular marker.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
103
118
https://svu.journals.ekb.eg/article_197059_27a3087a553bd3ba9d8cf1207758b116.pdf
dx.doi.org/10.21608/svu.2021.80987.1130
An Abattoir-based survey on the prevalence of some Gastrointestinal helminths of camels (Camelus dromedarius) in Aswan Province, Egypt
Mahmoud A.
El-Seify
Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
author
Ismail S.
Elshahawy
Department of Parasitology, Faculty of Veterinary Medicine, South Valley University, Egypt.
author
Omima
Ibrahim
Department of Parasitology, Animal Health Research Institute, Giza, Egypt
author
Zienab K.
Ahamed
Department of Parasitology, Animal Health Research Institute (Aswan Branch), Egypt
author
text
article
2021
eng
Gastrointestinal parasitic infection is a prime cause of failures in camel production. This study was conducted to ascertain the prevalence of gastrointestinal helminths of camels in Aswan district, Egypt. Besides, the prevalence of helminthiasis in camel was also evaluated considering age, gender, and rearing system. For such reason, a total of 110 dromedaries (Camelus dromedarius) in the Draw abattoir, Aswan province were screened for the presence of gastrointestinal helminths by postmortem examinations between December 2018 to November 2019. Twenty-four animals (21.82 %) were found to be infected with one or more species of gastrointestinal helminths. Moniezia expansa, and Stilesia globipunctata, emerged as the most prevalent species (29.2%), with Avitellina centripunctata also being common (20.83%). Whereas, Moniezia benedeni, was present at low infection rates (12.5 %). Furthermore, the only recorded trematode worm was Paramphistomes spp. (8.33%). Gender variations revealed significant (P≤0.05) differences among examined camels. Oppositely, age and seasonal dynamics had no significant differences with the highest peak in the winter season (33.3%). The current prevalence indicates the necessity of using an anthelmintic drug for increasing the health and productivity of camels.
SVU-International Journal of Veterinary Sciences
South Valley Univesity, Faculty of Veterinary Medicine
2535-1826
4
v.
3
no.
2021
119
130
https://svu.journals.ekb.eg/article_197169_f2f8ec2d10e1b8cc2fa7fab442d786cf.pdf
dx.doi.org/10.21608/svu.2021.83625.1133