Document Type : Research article
Authors
1
Department of Biochemistry, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt
2
Neurology and Psychological Medicine department, Faculty of Medicine, Sohag University
3
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
4
Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt (BUE), Cairo,
5
Poultry Research Department, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt
6
Department of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
7
Department of Pharmacology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
Abstract
The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascular- permeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX-2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway.
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