Molecular mechanism of Copper Albumin Complex on NDEA induced brain vascular damage via promoting VEGF expression.

Taha, Laila; Maher, Zainab M; Nassar, Ahmed Y; Abdallah, Mohamed S; Embark, Hamdy; Youssef, Mohammed; Abdeen, Ahmed; Moftah, Rofida F.; Madhyastha, Harishkumar; Shanab, Obeid

doi:10.21608/svu.2022.133515.1192

Molecular mechanism of Copper Albumin Complex on NDEA induced brain vascular damage via promoting VEGF expression.

Document Type : Research article

Authors

¹ biochemistry,faculty of veterinary medicine,south valley univercity,qena,egypt

² Department of Pathology and clinical pathology, Faculty of veterinary medicine, Qena university

³ 3Department of Medical Biochemistry and Molecular biology, Faculty of Medicine, Assiut University, Assiut, Egypt.

⁴ Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

⁵ Department of Animal Physiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt

⁶ Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.Center of Excellence for Screening of Environmental Contaminants (CESEC), Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.

⁷ Food Science and Technology Department, Faculty of Agriculture, Assiut University, Assiut, Egypt.

⁸ Department of Cardiovascular Physiology, Faculty of medicine, University of Miyazaki, Miyazaki 8891692, Japan.

⁹ Department of Biochemistry, faculty of veterinary medicine, South Valley University, Egypt.

10.21608/svu.2022.133515.1192

Abstract

Nitrosodiethylamine (NDEA) is a potent oxidant induces neurodegeneration via (reactive oxgen species) ROS. Copper is an important metal essential for scavenging free radicals, development of central nervous system (CNS) and redox angiogenesis signaling. Vascular endothelial growth factor (VEGF) is well known as efficacious and long-term signal that stimulates angiogenesis, where its expression is copper dependent.We examined thecopper protective effect against brain vascular damage initiated by NDEA. NDEA induces brain vascular wall damage, necrosis with interstitial hemorrhage and diminishesVEGF expression. Histopathological examination showing a great improvement of brain tissue in copper treated mice with significant increase in VEGF expression.Higher levels of intracellular copper can stimulate angiogenesis and exhibited a significant protection against NDEA induced brain vascular damage,confirming its ability to enhanceantioxidant activity andangiogenesis initiation.Our report presents first evidence that inducible VEGF expression in brain is sensitive to copper; moreover Copper based therapeutics represents a novel approach to reducebrain vascular damage induced by NDEA generated ROS.

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