Quality assessment and detection of multiple drug-resistant food-borne aerobic bacteria in frozen quail in Luxor and Aswan city

Ahmed, Zeinab; Elsayed, Huda; Albarbary, Nady Khairy

doi:10.21608/svu.2022.145199.1207

Quality assessment and detection of multiple drug-resistant food-borne aerobic bacteria in frozen quail in Luxor and Aswan city

Document Type : Research article

Authors

¹ Department of Food Hygiene, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Luxor, Egypt,

² Reference Lab for Safety Analysis of Food of Aimal Origin, Animal Health Research Institute, Agriculture Research Center, Giza, Egypt.

³ Department of Food Hygiene, Faculty of Veterinary Medicine, Aswan University

10.21608/svu.2022.145199.1207

Abstract

Quail meat is a sweet and delicate white game meat with extremely low skin fat and low cholesterol value; Fifty random samples were collected from different restaurants in Luxor and Aswan city, Egypt to evaluate the quality of frozen wild quail meat. The investigation revealed that (8%), (8%), and (12 %) of the examined samples were contaminated with E.coli, Staphylococcus aureus, and Salmonella spp., respectively as well as the mean values of APC, Coliform, and S. aureus counts were 4.4×104±0.074, 2.2×103±0.094 and 1.2×10±1.1 respectively. Serotyping revealed that the investigated E. coli isolates belonged to 3 different O-serogroups comprising O125 (50%), O55 (25%), and O86a (25%) while the examined Salmonella isolates including Salmonella Othmarschen (16.6%), Salmonella Livingstone (16.6%), Salmonella Kentucky (16.6%), Salmonella Tado (16.6%) and Salmonella enterica Subspecies Salamae (33.3%). Antimicrobial susceptibility testing for E. coli isolates revealed that they were sensitive to Colistin sulfate, Nalidixic acid, and Ceftriaxone while they were resistant to Gentamycin, Streptomycin. S. aureus isolates were sensitive to Ampicillin and Vancomycin while resistant to Erythromycin, Chloramphenicol, and Tetracycline. In addition, Salmonella isolates were sensitive to Amoxicillin and Streptomycin while resistant to Colistin sulfate. Moreover, the mean values of pH, total basic nitrogen (TVB-N mg/100gm), and Thiobarbituric acid (TBA mg/Kg) were 5.9±0.01, and 12.1±0.2 and 0.72±0.03, respectively. The results were statistically evaluated. The possible sources of quail meat contamination, the Public health importance of the isolated bacteria, and the hygienic measures which should be imposed were discussed.

Keywords