Scale up of Xylanase Production by Submerged Fermentation from Brevibacillus Borstelentis (MTCC 9874) and Digestibility Assessment of Crude Xylanase in Wheat.

Document Type : Research article

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Pharmaceutical Biotechnology Research Lab, Department of Pharmacy, School of Science, Kathmandu University, Dhulikhel, Kavre, GPO Box 6250 (Kathmandu), Nepal

Abstract

       The main objective of this study was to scale up xylanase production from isolated soil microbe (Brevibacillus borselensis; the Microbial Type Culture Collection (MTCC) 9874) in a bioreactor and check digestibility of this crude enzyme in animal feed (wheat). Scale up was done from lab production Xylanolytic activity in bioreactor is greater than flask method due to more controlled condition in bioreactor which is not possible in flask method. Digestibility of crude enzyme was checked in wheat and was found that 16 (15.797) times digestion of wheat animal feed will be increased if it is supplemented with crude xylanase produced in bioreactor in the given condition. Although, pure enzymes are very expensive, this may not be affordable for farmers. Crude enzymes can be produced locally, or it will be very cheap and of low cost as it is present in the crude form thus, it is considered of a great value to farmers to increase their productivity and change their socio-economic condition

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